Metachromatic Agar-Diffusion Methods for Detecting Staphylococcal Nuclease Activity
نویسندگان
چکیده
منابع مشابه
Metachromatic agar-diffusion methods for detecting staphylococcal nuclease activity.
Based on the metachromatic property of Toluidine Blue O, three, convenient agar-diffusion methods have been developed that enable detection of the nuclease of Staphylococcus aureus at concentrations as low as 0.005 mug/ml in agar and broth cultures. The interactions of agar and deoxyribonucleic acid with Toluidine Blue O are discussed.
متن کاملConvenient assay for staphylococcal nuclease by the metachromatic well-agar-diffusion technique.
The metachromatic agar-diffusion (MAD) microslide technique was adapted for quantitative assay for staphylococcal thermonuclease in heterogeneous systems, such as milk and broth. When an enzyme-containing solution was placed in a well cut in the agar, a bright pink halo was obtained. The diameter of the pink zone of hydrolysis was related to time and temperature of incubation and to nuclease co...
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Background and Objective: Nowadays, incidence of antibiotic-resistance among pathogenic bacteria has increased due to indiscriminate use of antimicrobial drugs for treatment of diseases, especially urinary tract infections. Medicinal plants are also of great importance as antibacterial agents. Therefore, the aim of this study was to determine the antibacterial effect of ethanolic extract of n...
متن کامل[Cold denaturation of staphylococcal nuclease].
It has been shown that the structure of staphylococcal nuclease breaks down reversibly both at a temperature increase above 20 degrees C and at its decrease. Both the heat and cold denaturations of protein are well approximated by a transition between two states differing in heat capacity, which means that the whole protein molecule represents a unique cooperative system with a well developed h...
متن کاملevaluation of antibacterial activity of urtica dioica l. leaf ethanolic extract using agar well diffusion and disc diffusion methods
background and objective: nowadays, incidence of antibiotic-resistance among pathogenic bacteria has increased due to indiscriminate use of antimicrobial drugs for treatment of diseases, especially urinary tract infections. medicinal plants are also of great importance as antibacterial agents. therefore, the aim of this study was to determine the antibacterial effect of ethanolic extract of net...
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ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1971
ISSN: 0003-6919
DOI: 10.1128/aem.21.4.585-587.1971